| Product name | Nanog P8 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the Internal region of human Nanog P8 at AA range: 30-110 |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB,IF,ELISA,IHC |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;IF/ICC1:50-200;ELISA 1:2000-20000;IHC 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | NANOGP8; Putative homeobox protein NANOGP8 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 35kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | This locus is a processed pseudogene of the transcription factor NANOG. NANOG plays a central role in regulating self-renewal in pluripotent stem cells and tumor cells. This pseudogene contains an intact open reading frame that could potentially encode a protein similar to NANOG. Although there is no evidence of transcription from this pseudogene, RT-PCR studies suggest that NANOGP8 may be expressed in some cancer cell lines. In vitro studies using a recombinant NANOGP8 protein have shown that the protein localizes to the nucleus and can promote cell proliferation, similar to NANOG. |
| Gene ID | 388112 |
| Alternative | NANOGP8; Putative homeobox protein NANOGP8 |
| Others | Nanog P8 Polyclonal Antibody detects endogenous levels of Nanog P8 protein. |
| Accession | Q6NSW7 |
| Observed Band(KD) | 35 |
Fig. Western Blot analysis of various cells using Nanog P8 Polyclonal Antibody diluted at 1:2000.
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