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Myosin Heavy Chain Monoclonal Antibody

Myosin Heavy Chain Monoclonal Antibody

Views(400) Publications(0) Catalog no(ABM40058)
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Specification

Product name Myosin Heavy Chain Monoclonal Antibody
Immunogen Synthetic Peptide
Host Mouse
Reactivity Fruit Fly, Human, Mouse, Nematode, Rat
Applications IF
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF (1:100).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Alternative Myosin Heavy Chain

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Skeletal muscle Myosin or myosin II is the motor protein that generates force to drive muscle contraction. It is a 520 kDa hexamer comprised of two heavy chains and four light chains. Myosin heavy chain is 220 kDa in size and consists of a long coiled-coil domain tail that mediates dimerization of the two heavy chains and a globular head region that mediates ATP-dependent sliding of actin filaments. Myosin heavy chain can be proteolytically cleaved to produce heavy meromyosin, which includes the S1 motor domain (head region) and first third of the coiled coil domain, and light meromyosin, which includes the C-terminal two thirds of the coiled coil domain.
Alternative Myosin Heavy Chain
Others The antibody detects endogenous MHC proteins.
Observed Band(KD) 220

Image & description

Fig. Immunofluorescence Staining of nematode tissue with MYH Mouse mAb (11C2) diluted at 1:100 (Provide by Tsinghua University) .

Fig. Immunofluorescence Staining of nematode tissue with MYH Mouse mAb (11C2) diluted at 1:100 (Provide by Tsinghua University) .

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