| Product name | MyoD Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from human MyoD around the non-acetylation site of K99/K102 |
| Host | Rabbit |
| Reactivity | Chicken, Human, Mouse, Rat |
| Applications | ELISA, WB |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:20000). Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Alternative | MYOD1; BHLHC1; MYF3; MYOD; Myoblast determination protein 1; Class C basic helix-loop-helix protein 1; bHLHc1; Myogenic factor 3; Myf-3 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Storage buffer | PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | MYOD1 encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. |
| Gene ID | 4654 |
| Alternative | MYOD1; BHLHC1; MYF3; MYOD; Myoblast determination protein 1; Class C basic helix-loop-helix protein 1; bHLHc1; Myogenic factor 3; Myf-3 |
| Others | MyoD Polyclonal Antibody detects endogenous levels of MyoD protein. |
| Accession | P15172 |
| Observed Band(KD) | 35 |
Fig. Western Blot analysis of Jurkat cells using MyoD Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Author：M Ma, B Cai, Z Zhou, S Kong, J Zhang, H Xu Publication name：Cell Communication and Signaling IF：8.4
Author：Zhang, Zihao Publication name：International Journal of Biological Macromolecules IF：8.192
Author：Cai B, Ma M, Zhou Z Publication name：J Anim Sci Biotechnol IF：4.167
Author：B Cai, Z Li, M Ma, J Zhang Publication name：Molecular Therapy Nucleic Acids IF：
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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