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MYH-pan Polyclonal Antibody

MYH-pan Polyclonal Antibody

Views(90) Publications(0) Catalog no(ABP53158)
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Specification

Product name MYH-pan Polyclonal Antibody
Immunogen Synthesized peptide derived from human MYH-pan around the non-acetylation site of K1394
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative MYH1; Myosin-1; Myosin heavy chain 1; Myosin heavy chain 2x; MyHC-2x; Myosin heavy chain IIx/d; MyHC-IIx/d; Myosin heavy chain, skeletal muscle, adult 1; MYH2; MYHSA2; Myosin-2; Myosin heavy chain 2Myosin heavy chain 2; Myosin heavy chain 2a; MyHC-2a; Myosin heavy chain IIa; MyHC-IIa; Myosin heavy chain, skeletal muscle, adult 2; MYH3; Myosin-3; Muscle embryonic myosin heavy chain; Myosin heavy chain 3

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development.
Gene ID 4619/4620/4621/5795/4624/4625/4626
Alternative MYH1; Myosin-1; Myosin heavy chain 1; Myosin heavy chain 2x; MyHC-2x; Myosin heavy chain IIx/d; MyHC-IIx/d; Myosin heavy chain, skeletal muscle, adult 1; MYH2; MYHSA2; Myosin-2; Myosin heavy chain 2Myosin heavy chain 2; Myosin heavy chain 2a; MyHC-2a; Myosin heavy chain IIa; MyHC-IIa; Myosin heavy chain, skeletal muscle, adult 2; MYH3; Myosin-3; Muscle embryonic myosin heavy chain; Myosin heavy chain 3
Others MYH-pan Polyclonal Antibody detects endogenous levels of MYH-pan protein only when non-acetylation at K1394.
Accession P12882/Q9UKX2/P11055/Q9Y623/P13533/P12883/P13535
Observed Band(KD) 225

Image & description

Fig.1. Western Blot analysis of 4T1 cells using MYH-pan Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western Blot analysis of 4T1 cells using MYH-pan Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-muscle, antibody was diluted at 1:100.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-muscle, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-muscle, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-muscle, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded Mouse-muscle, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded Mouse-muscle, antibody was diluted at 1:100.

Fig.5. Immunohistochemical analysis of paraffin-embedded Mouse-muscle, antibody was diluted at 1:100.

Fig.5. Immunohistochemical analysis of paraffin-embedded Mouse-muscle, antibody was diluted at 1:100.

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