| Product name | MRP-L35 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the Internal region of human MRP-L35 at AA range: 80-160 |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | ELISA, WB |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Alternative | MRPL35; BM-007; 39S ribosomal protein L35, mitochondrial; L35mt; MRP-L35 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Storage buffer | PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes(mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPL35(mitochondrial ribosomal protein L35) encodes a 39S subunit protein. Sequence analysis identified three transcript variants. Pseudogenes corresponding to MRPL35 are found on chromosomes 6p, 10q, and Xp. |
| Gene ID | 51318 |
| Alternative | MRPL35; BM-007; 39S ribosomal protein L35, mitochondrial; L35mt; MRP-L35 |
| Others | MRP-L35 Polyclonal Antibody detects endogenous levels of MRP-L35 protein. |
| Accession | Q9NZE8 |
| Observed Band(KD) | 21 |
Fig.1. Western blot analysis of 293T COLO using MRP-L35 antibody. Antibody was diluted at 1:2000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Western blot analysis of 293 using MRP-L35 antibody. Antibody was diluted at 1:2000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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