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MPO Polyclonal Antibody

MPO Polyclonal Antibody

Views(4) Publications(0) Catalog no(ABP53082)
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Specification

Product name MPO Polyclonal Antibody
Immunogen Synthesized peptide derived from the N-terminal region of human MPO
Host Rabbit
Reactivity Human,Mouse,Rat
Applications WB,IHC,IF,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC: 100-300;WB 1:500-1:2000;ELISA 1:20000;IF 1:50-200
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative MPO; Myeloperoxidase; MPO

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 85kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils.
Gene ID 4353
Alternative MPO; Myeloperoxidase; MPO
Others MPO Polyclonal Antibody detects endogenous levels of MPO protein.
Accession P05164
Observed Band(KD) 85

Image & description

Fig. Western Blot analysis of K562 cells using MPO Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig. Western Blot analysis of K562 cells using MPO Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

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