|Product name||Mouse Vasohibin-1 (VASH1) ELISA Kit|
|Applications notes||This Mouse Vasohibin-1 (VASH1) ELISA Kit employs a two-site sandwich ELISA to quantitate VASH1 in samples. An antibody specific for VASH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVASH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VASH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VASH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Mouse Vasohibin-1 microplate
• Mouse Vasohibin-1 standard
• Mouse Vasohibin-1 detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Mouse Vasohibin-1 (VASH1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse VASH1. No significant cross-reactivity or interference between Mouse VASH1 and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||VASH2 deduced 355-amino acid protein shares 52.5% amino acid identity with VASH1 and 97.5% amino acid identity with mouse Vash2. VASH2 expression at embryonic weeks 6 to 12 in human heart, brain, kidney, lung, muscle, and whole embryo. RT-PCR analysis detected low levels of VASH2 expression in HUVECs and aortic endothelial cells. VASH2 expression in cell lines was not inducible by VEGF, FGF2, or PMA, all of which can induce VASH1 expression. However, in an in vivo mouse model, Vash2 was induced in embryonic brain transfected with VEGF. Immunohistochemistry showed that VASH2 and VASH1 were present in vessels from 20-week human embryonic lung.VASH2 has antiangiogenic activity. In addition, VASH2 inhibited network formation by HUVECs.|
Fig.1. Mouse Vasohibin-1 (VASH1) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
You must be logged in to post a review.
1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
Welcome any form of communications, and better service will be provided here.
Address： Bldg C17, Optics Valley International Biomedicine Park, Wuhan, China. 430223
There are no reviews yet.