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Mouse Tumor necrosis factor ligand superfamily member 12 (TNFSF12) ELISA Kit

Mouse Tumor necrosis factor ligand superfamily member 12 (TNFSF12) ELISA Kit

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Specification

Product name Mouse Tumor necrosis factor ligand superfamily member 12 (TNFSF12) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Tumor necrosis factor ligand superfamily member 12 (TNFSF12) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFSF12 in samples. An antibody specific for TNFSF12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFSF12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFSF12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFSF12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TNFSF12; APO3L; DR3LG; MGC129581; MGC20669; TWEAK; APO3/DR3 ligand; TNF-related WEAK inducer of apoptosis

Product Properties

Kit components • Mouse Tumor necrosis factor ligand superfamily member 12 microplate
• Mouse Tumor necrosis factor ligand superfamily member 12 standard
• Mouse Tumor necrosis factor ligand superfamily member 12 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Tumor necrosis factor ligand superfamily member 12 (TNFSF12) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNFSF12. No significant cross-reactivity or interference between Mouse TNFSF12 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Tumor necrosis factor ligand superfamily member 12 is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis.The deduced in-frame fusion protein, which the authors called TWEPRIL, has a calculated molecular mass of 38 kD. TWEPRIL begins at the initiation codon of TWEAK and contains the intracellular, transmembrane, and stalk regions of TWEAK fused to the extracellular receptor-binding domain of APRIL.
Gene ID 21944
Alternative TNFSF12; APO3L; DR3LG; MGC129581; MGC20669; TWEAK; APO3/DR3 ligand; TNF-related WEAK inducer of apoptosis
Accession O54907

Image & description

Fig.1. Mouse Tumor necrosis factor ligand superfamily member 12 (TNFSF12) Standard Curve.

Fig.1. Mouse Tumor necrosis factor ligand superfamily member 12 (TNFSF12) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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