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Mouse Trinucleotide repeat-containing gene 6B protein (TNRC6B) ELISA Kit

Mouse Trinucleotide repeat-containing gene 6B protein (TNRC6B) ELISA Kit

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Specification

Product name Mouse Trinucleotide repeat-containing gene 6B protein (TNRC6B) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Trinucleotide repeat-containing gene 6B protein (TNRC6B) ELISA Kit employs a two-site sandwich ELISA to quantitate TNRC6B in samples. An antibody specific for TNRC6B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNRC6B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNRC6B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNRC6B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TNRC6B; RP5-1042K10.7; KIAA1093; OTTHUMP00000199175

Product Properties

Kit components • Mouse Trinucleotide repeat-containing gene 6B protein microplate
• Mouse Trinucleotide repeat-containing gene 6B protein standard
• Mouse Trinucleotide repeat-containing gene 6B protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Trinucleotide repeat-containing gene 6B protein (TNRC6B) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNRC6B. No significant cross-reactivity or interference between Mouse TNRC6B and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The deduced 1,203-amino acid protein contains an RNA recognition motif. RT-PCR ELISA detected moderate expression in all tissues and specific brain regions examined, with highest expression in cerebellum. Schneider et al. (2006) stated that the 1,727-amino acid TNRC6B protein contains a glycine/tryptophan (GW)-rich N-terminal domain, a central glutamine-rich region, and a C-terminal RNA recognition motif. TNRC6B copurified with a cytoplasmic HeLa cell protein complex containing AGO2 (EIF2C2), DICER, and MOV10. Epitope-tagged TNRC6B coimmunoprecipitated with tagged AGO1 (EIF2C1) and tagged AGO2. TNRC6B was required to mediate microRNA-guided mRNA cleavage in HeLa cell culture.
Gene ID 213988
Alternative TNRC6B; RP5-1042K10.7; KIAA1093; OTTHUMP00000199175
Accession Q8BKI2

Image & description

Fig.1. Mouse Trinucleotide repeat-containing gene 6B protein (TNRC6B) Standard Curve.

Fig.1. Mouse Trinucleotide repeat-containing gene 6B protein (TNRC6B) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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