| Product name | Mouse Transmembrane protein 55B (TMEM55B) ELISA Kit |
| Reactivity | Mouse |
| Applications | ELISA |
| Applications notes | This Mouse Transmembrane protein 55B (TMEM55B) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM55B in samples. An antibody specific for TMEM55B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM55B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM55B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM55B bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | TMEM55B; C14orf9; DKFZp434M0519; MGC26684; PtdIns-4;5-P(2) 4-phosphatase type I |
| Kit components | • Mouse Transmembrane protein 55B microplate • Mouse Transmembrane protein 55B standard • Mouse Transmembrane protein 55B detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Mouse Transmembrane protein 55B (TMEM55B) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMEM55B. No significant cross-reactivity or interference between Mouse TMEM55B and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | TMEM55B catalyzes the degradation of phosphatidylinositol 4,5-bisphosphate (PtdIns-4,5-P2) by removing the 4-phosphate. The deduced 257-amino acid protein contains a central Cx(5)R phosphatase catalytic motif and 2 transmembrane domains near its C terminus, a characteristic of lysosomal transmembrane proteins. RNA dot blot analysis detected expression in all tissues examined. Fluorescence-tagged TMEM55B localized with membrane markers of late endosomes or lysosomes. Endogenous HeLa cell TMEM55B showed a similar distribution.It showed no activity toward other phosphatidylinositol substrates or inositol phosphates tested. Overexpression of TMEM55B in human embryonic kidney cells reduced the total cellular level of PtdIns-4,5-P2. |
| Gene ID | 219024 |
| Alternative | TMEM55B; C14orf9; DKFZp434M0519; MGC26684; PtdIns-4;5-P(2) 4-phosphatase type I |
| Accession | Q3TWL2 |
Fig.1. Mouse Transmembrane protein 55B (TMEM55B) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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