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Mouse Transmembrane and ubiquitin-like domain-containing protein 1 (TMUB1) ELISA Kit

Mouse Transmembrane and ubiquitin-like domain-containing protein 1 (TMUB1) ELISA Kit

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Specification

Product name Mouse Transmembrane and ubiquitin-like domain-containing protein 1 (TMUB1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Transmembrane and ubiquitin-like domain-containing protein 1 (TMUB1) ELISA Kit employs a two-site sandwich ELISA to quantitate TMUB1 in samples. An antibody specific for TMUB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMUB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMUB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMUB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TMUB1; C7orf21; MGC5442; SB144;

Product Properties

Kit components • Mouse Transmembrane and ubiquitin-like domain-containing protein 1 microplate
• Mouse Transmembrane and ubiquitin-like domain-containing protein 1 standard
• Mouse Transmembrane and ubiquitin-like domain-containing protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Transmembrane and ubiquitin-like domain-containing protein 1 (TMUB1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMUB1. No significant cross-reactivity or interference between Mouse TMUB1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Tmub1 encodes for a protein, HOPS/DULP, containing transmembrane domains and a ubiquitin-like domain (UBL) Ubiquitin can be employed to modify proteins by either single or multiple ubiquitin conjugation to mark them for proteasomal destruction, endocytosis and binding to other proteins. Tmub1/HOPS was reported to facilitate the recycling of AMPA receptors into synaptic membrane in cultured primary neurons. Tmub1/HOPS was also found overexpressed during liver regeneration and is an essential constituent of centrosome assembly during cell cycles in culture. Like many UBL domain proteins, the physiological and molecular function of Tmub1 is far from being clear. The resulting mice Tmub1were put through an extensive battery of assays to discover physiological and behavioral phenotypes.
Gene ID 64295
Alternative TMUB1; C7orf21; MGC5442; SB144;
Accession Q9JMG3

Image & description

Fig.1. Mouse Transmembrane and ubiquitin-like domain-containing protein 1 (TMUB1) Standard Curve.

Fig.1. Mouse Transmembrane and ubiquitin-like domain-containing protein 1 (TMUB1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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