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Mouse Toll-like receptor 1 (TLR1) ELISA Kit

Mouse Toll-like receptor 1 (TLR1) ELISA Kit

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Specification

Product name Mouse Toll-like receptor 1 (TLR1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Toll-like receptor 1 (TLR1) ELISA Kit employs a two-site sandwich ELISA to quantitate TLR1 in samples. An antibody specific for TLR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTLR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TLR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TLR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TLR1; CD281; DKFZp547I0610; DKFZp564I0682; KIAA0012; MGC104956; MGC126311; MGC126312; TIL; rsc786; Toll/interleukin-1 receptor-like

Product Properties

Kit components • Mouse Toll-like receptor 1 microplate
• Mouse Toll-like receptor 1 standard
• Mouse Toll-like receptor 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Toll-like receptor 1 (TLR1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TLR1. No significant cross-reactivity or interference between Mouse TLR1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background TLR 1 is a member of the Toll-like receptor family (TLR) of pattern recognition receptors of the innate immune system. TLR1 recognizes pathogen-associated molecular pattern with a specificity for gram-positive bacteria. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is ubiquitously expressed, and at higher levels than other TLR genes. Different length transcripts presumably resulting from use of alternative polyadenylation site, and/or from alternative splicing, have been noted for this gene.
Gene ID 21897
Alternative TLR1; CD281; DKFZp547I0610; DKFZp564I0682; KIAA0012; MGC104956; MGC126311; MGC126312; TIL; rsc786; Toll/interleukin-1 receptor-like
Accession Q9EPQ1

Image & description

Fig.1. Mouse Toll-like receptor 1 (TLR1) Standard Curve.

Fig.1. Mouse Toll-like receptor 1 (TLR1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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