|Product name||Mouse Spermatid nuclear transition protein 1 (TNP1) ELISA Kit|
|Applications notes||This Mouse Spermatid nuclear transition protein 1 (TNP1) ELISA Kit employs a two-site sandwich ELISA to quantitate TNP1 in samples. An antibody specific for TNP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Mouse Spermatid nuclear transition protein 1 microplate
• Mouse Spermatid nuclear transition protein 1 standard
• Mouse Spermatid nuclear transition protein 1 detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Mouse Spermatid nuclear transition protein 1 (TNP1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNP1. No significant cross-reactivity or interference between Mouse TNP1 and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Transition protein-1 is a spermatid-specific product of the haploid genome which replaces histone and is itself replaced in the mature sperm by the protamines. Yu et al. (2000) generated mice lacking the major transition nuclear protein, TP1, by targeted deletion of the Tnp1 gene in mouse embryonic stem cells. Surprisingly, testis weights and sperm production were normal in the mutant mice, and only subtle abnormalities were detected in sperm morphology. Electron microscopy showed large rod-like structures in the chromatin of mutant step 13 spermatids, in contrast to the fine chromatin fibrils observed in wildtype. Steps 12-13 spermatid nuclei from the testis of Tnp1- mice contained, in place of TP1, elevated levels of TP2 (TNP2) and some PRM2 precursor.|
Fig.1. Mouse Spermatid nuclear transition protein 1 (TNP1) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.