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Mouse Speckle-type POZ protein-like (SPOPL) ELISA Kit

Mouse Speckle-type POZ protein-like (SPOPL) ELISA Kit

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Specification

Product name Mouse Speckle-type POZ protein-like (SPOPL) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Speckle-type POZ protein-like (SPOPL) ELISA Kit employs a two-site sandwich ELISA to quantitate SPOPL in samples. An antibody specific for SPOPL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPOPL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPOPL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPOPL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SPOPL; FLJ53775; HIB homolog 2; roadkill homolog 2

Product Properties

Kit components • Mouse Speckle-type POZ protein-like microplate
• Mouse Speckle-type POZ protein-like standard
• Mouse Speckle-type POZ protein-like detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Speckle-type POZ protein-like (SPOPL) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SPOPL. No significant cross-reactivity or interference between Mouse SPOPL and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background SPOPL Belongs to the Tdpoz family.Contains 1 BTB (POZ) domain.Contains 1 MATH domain.TDPOZ, a family of bipartite animal and plant proteins that contain the TRAF (TD) and POZ/BTB domains. The Tdpoz gene cluster is mapped on chromosome 3 at 3F2.1-2.2. RT-PCR experiments and a search of expressed sequence tag (EST) databases show that the Tdpoz1-5 genes are transcribed in early embryos, particularly at the two-cell stage. TDPOZ proteins form a new protein family on the basis of similar protein domain organization. TDPOZ proteins may be nuclear scaffold proteins probably involved in transcription regulation in early development and other cellular processes.
Gene ID 76857
Alternative SPOPL; FLJ53775; HIB homolog 2; roadkill homolog 2
Accession Q2M2N2

Image & description

Fig.1. Mouse Speckle-type POZ protein-like (SPOPL) Standard Curve.

Fig.1. Mouse Speckle-type POZ protein-like (SPOPL) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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