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Mouse Serine palmitoyltransferase 2 (SPTLC2) ELISA Kit

Mouse Serine palmitoyltransferase 2 (SPTLC2) ELISA Kit

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Specification

Product name Mouse Serine palmitoyltransferase 2 (SPTLC2) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Serine palmitoyltransferase 2 (SPTLC2) ELISA Kit employs a two-site sandwich ELISA to quantitate SPTLC2 in samples. An antibody specific for SPTLC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPTLC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPTLC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPTLC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SPTLC2; KIAA0526; LCB2; SPT2; serine palmitoyltransferase; subunit

Product Properties

Kit components • Mouse Serine palmitoyltransferase 2 microplate
• Mouse Serine palmitoyltransferase 2 standard
• Mouse Serine palmitoyltransferase 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Serine palmitoyltransferase 2 (SPTLC2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SPTLC2. No significant cross-reactivity or interference between Mouse SPTLC2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Serine palmitoyltransferase (SPT; EC 2.3.1.50) is the key enzyme in sphingolipid biosynthesis. It catalyzes the pyridoxal-5-prime-phosphate-dependent condensation of L-serine and palmitoyl-CoA to 3-oxosphinganine. SPTLC2encodes a long chain base subunit of serine palmitoyltransferase. Serine palmitoyltransferase, which consists of two different subunits, is the initial enzyme in sphingolipid biosynthesis. It catalyzes the pyridoxal 5'-phosphate dependent condensation of L-serine and palmitoyl CoA to 3-oxosphinganine. Mutations in this gene were identified in patients with hereditary sensory neuropathy type I. Alternatively spliced variants encoding different isoforms have been identified.The LCB2 cDNA encodes a protein of 562 amino acids that is more than 90% identical to the mouse protein.
Gene ID 20773
Alternative SPTLC2; KIAA0526; LCB2; SPT2; serine palmitoyltransferase; subunit
Accession P97363

Image & description

Fig.1. Mouse Serine palmitoyltransferase 2 (SPTLC2) Standard Curve.

Fig.1. Mouse Serine palmitoyltransferase 2 (SPTLC2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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