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Mouse/Rat TGF beta 1, His tag (Animal-Free)

Mouse/Rat TGF beta 1, His tag (Animal-Free)

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Specification

Product name Mouse/Rat TGF beta 1, His tag (Animal-Free)
Sequence Amino acid sequence derived from Mouse/Rat TGF beta 1 (Ala279-Cys390) (Mouse:P04202, Rat:P17246) was expressed with 6×His tag at the C-terminus
Applications His tag
Applications notes Mouse/Rat
Activity Measure by its ability to inhibit the IL-4 dependent proliferation in HT-2 cells.The ED₅₀ for this effect is <0.1 ng/mL.
Protein length The recombinant Mouse/Rat TGF beta 1 consists of 112 amino acids and predicts a molecular mass of 13.8 kDa
Preparation method E. coli
Purity > 98% as determined by SDS-PAGE.
Alternative Differentiation inhibiting factor, Cartilage-inducing factor, Tgfb-1

Product Properties

Formulation The protein was lyophilized from sterile 20 mM sodium citrate, 0.2 M NaCl, pH 3.5. If you have any concerns or special requirements, please confirm with us.
Features & Benefits Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.
Molecular weight 13.8 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously
Storage instructions Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles
Shipping The product is shipped with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The transforming Growth Factors beta (TGF beta) family of cytokines are ubiquitous, multifunctional, and essential to survival. They play the central roles in growth, development, inflammation, repair, and host immunity. The mammalian TGF beta isoforms (TGF beta 1, beta 2 and beta 3) are secreted as potential precursors and possess a variety of cell surface receptors and produces at least two mediate signal transductions.
Alternative Differentiation inhibiting factor, Cartilage-inducing factor, Tgfb-1
Accession P04202(Mouse), P17246(Rat)

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