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Mouse Prolargin (PRELP) ELISA Kit

Mouse Prolargin (PRELP) ELISA Kit

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Specification

Product name Mouse Prolargin (PRELP) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Prolargin (PRELP) ELISA Kit employs a two-site sandwich ELISA to quantitate PRELP in samples. An antibody specific for PRELP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRELP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRELP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRELP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PRELP; MGC45323; MST161; MSTP161; SLRR2A; 55 kDa leucine-rich repeat protein of articular cartilage; prolargin proteoglycan; proline arginine-rich end leucine-rich repeat protein

Product Properties

Kit components • Mouse Prolargin microplate
• Mouse Prolargin standard
• Mouse Prolargin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Prolargin (PRELP) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PRELP. No significant cross-reactivity or interference between Mouse PRELP and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PREPL belongs to the prolyl oligopeptidase subfamily of serine peptidases. Mutations in this gene have been associated with hypotonia-cystinuria syndrome, also known as the 2p21 deletion syndrome. Several alternatively spliced transcript variants encoding either the same or different isoforms have been described for this gene. The longest deduced protein contains 727 amino acids and has a propeller domain in its N-terminal half and a catalytic domain, including the catalytic triad (ser, asp, his) essential for serine peptidases, in its C-terminal half. In sharp contrast to PREP and oligopeptidase B, which require both amino- and carboxy-terminal sequences for activity, PREPL activity appeared to depend only on the carboxy-terminal domain.
Gene ID 116847
Alternative PRELP; MGC45323; MST161; MSTP161; SLRR2A; 55 kDa leucine-rich repeat protein of articular cartilage; prolargin proteoglycan; proline arginine-rich end leucine-rich repeat protein
Accession Q9JK53

Image & description

Fig.1. Mouse Prolargin (PRELP) Standard Curve.

Fig.1. Mouse Prolargin (PRELP) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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