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Mouse Mitochondrial import receptor subunit TOM22 homolog (TOMM22) ELISA Kit

Mouse Mitochondrial import receptor subunit TOM22 homolog (TOMM22) ELISA Kit

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Specification

Product name Mouse Mitochondrial import receptor subunit TOM22 homolog (TOMM22) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Mitochondrial import receptor subunit TOM22 homolog (TOMM22) ELISA Kit employs a two-site sandwich ELISA to quantitate TOMM22 in samples. An antibody specific for TOMM22 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTOMM22 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOMM22 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOMM22 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TOMM22; 1C9-2; MST065; MSTP065; TOM22; mitochondrial import receptor Tom22

Product Properties

Kit components • Mouse Mitochondrial import receptor subunit TOM22 homolog microplate
• Mouse Mitochondrial import receptor subunit TOM22 homolog standard
• Mouse Mitochondrial import receptor subunit TOM22 homolog detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Mitochondrial import receptor subunit TOM22 homolog (TOMM22) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TOMM22. No significant cross-reactivity or interference between Mouse TOMM22 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The translocase of outer mitochondrial membrane (TOM) complex is a multisubunit complex involved in the recognition, unfolding, and translocation of preproteins from the cytosol into the mitochondria. The deduced 142-amino acid human protein, which is approximately 33% similar to the yeast proteins, contains an N-terminal negatively charged region, an internal hydrophobic transmembrane region, and a C-terminal region with a glutamine-rich segment. Immunoblot analysis and fluorescence microscopy showed expression of a 21-kD mitochondrial membrane protein with its N and C termini exposed to the cytosol and intermembrane space of the mitochondrial outer membrane, respectively.An association between the rat Tomm22 protein, which is 94% identical to the human protein, and Tomm40 was observed.
Gene ID 223696
Alternative TOMM22; 1C9-2; MST065; MSTP065; TOM22; mitochondrial import receptor Tom22
Accession Q9CPQ3

Image & description

Fig.1. Mouse Mitochondrial import receptor subunit TOM22 homolog (TOMM22) Standard Curve.

Fig.1. Mouse Mitochondrial import receptor subunit TOM22 homolog (TOMM22) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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