| Product name | Mouse IL-36γ Protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Mouse IL-36γ (Gly13-Ser164) (Q8R460) was expressed with 6×His tag at the C-terminus. |
| Applications | His tag |
| Applications notes | Mouse |
| Activity | Measure by its ability to induce IL-6 secretion in 3T3 cells. The ED₅₀ for this effect is <15 ng/mL. The specific activity of recombinant mouse IL-36 gamma is > 6 x 10⁴ IU/mg. |
| Protein length | The recombinant mouse IL-36γ consists of 152 amino acids and predicts a molecular mass of 18.27 kDa. |
| Preparation method | E.coli |
| Purity | > 98 % as determined by SDS-PAGE |
| Molecular Formula | 18.27 kDa |
| Alternative | interleukin 36 gamma, interleukin 1 family, member 9, IL-1F9, IL36g, IL36 |
| Formulation | Lyophilized from sterile PBS，pH 7.4. |
| Features & Benefits | <0.1 EU per μg of the protein as determined by the LAL method. |
| Molecular weight | 18.27 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously. |
| Storage instructions | Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA,10% FBS or 0.1%BSA). Please prevent freeze-thaw cycles. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Interleukin 36 gamma (IL-36 gamma) is a 17.65 kDa cytokine with 159 amino acid residues. IL-36 gamma is a member of the IL-1 family and is mainly expressed in keratinocytes, bronchial epithelial cells, macrophages, and monocyte. This cytokine responds to multiple pathogen-associated molecular patterns (PAMPs) and participates in the inflammatory response. In addition, it also induces the production of pro-inflammatory cytokines like IL-12, IL-1β, IL-6, TNF-α, and IL-23. |
| Gene ID | 215257 |
| Alternative | interleukin 36 gamma, interleukin 1 family, member 9, IL-1F9, IL36g, IL36 |
| Accession | Q8R460 |
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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