| Product name |
Mouse IL-3 Protein, His tag (Animal-Free) |
| Sequence |
Amino acid sequence derived from Mouse IL-3 (Asp33-Cys166) (P01586) was expressed with 6×His tag at the C-terminus and a Met at the N-terminus |
| Applications |
His tag |
| Applications notes |
Mouse |
| Activity |
Measure by its ability to induce NFS-60 cells proliferation.The ED₅₀ for this effect is <85 pg/mL.The specific activity of recombinant mouse IL-3 is approximately >1x 10⁷ IU/mg. |
| Protein length |
The recombinant Mouse IL-3 Protein consists of 134 amino acids and predicts a molecular mass of 16.04 kDa |
| Preparation method |
E. coli |
| Purity |
> 98% as determined by SDS-PAGE. |
| Alternative |
MCGF (Mast Cell Growth Factor), Multi-CSF, HCGF, P-cell stimulation factor, Interleukin-3b, BPA, PSF, Csfmu |
| Formulation |
The protein was lyophilized from sterile PBS, pH 7.4.. If you have any concerns or special requirements, please confirm with us. |
| Features & Benefits |
Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight |
16.04 kDa |
| Usage notes |
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously. |
| Storage instructions |
Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles. |
| Shipping |
The product is shipped with blue ice. |
| Precautions |
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background |
Interleukin-3 (IL-3) is a pleiotropic cytokine which can stimulates the survival, differentiation and proliferation of committed progenitor cells, including megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. IL-3 also enhances phagocytosis and antibody-mediated cellular cytotoxicity. IL-3 binds to IL-3R (IL-3 receptor), which is composed of a unique α subunit (IL-3Rα) and a common β-subunit (βc). |
| Alternative |
MCGF (Mast Cell Growth Factor), Multi-CSF, HCGF, P-cell stimulation factor, Interleukin-3b, BPA, PSF, Csfmu |
| Accession |
P01586 |
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