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Mouse IL-22 Protein, His tag (Animal-Free)

Mouse IL-22 Protein, His tag (Animal-Free)

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Specification

Product name Mouse IL-22 Protein, His tag (Animal-Free)
Sequence Amino acid sequence derived from Mouse IL-22 (Leu24-Val179) (Q9JJY9) was expressed with 6×His tag at the C-terminus.
Applications His tag
Applications notes Mouse
Activity Measure by its ability to induce IL-10 secretion in COLO205 cells.The ED₅₀ for this effect is <0.3 ng/mL.
Protein length The recombinant mouse IL-22 consists of 156 amino acids and predicts a molecular mass of 17.58 kDa.
Preparation method E.coli
Purity > 98 % as determined by SDS-PAGE
Molecular Formula 17.58 kDa
Alternative interleukin 22, IL-TIF, Cytokine ZCYTO18, If2b1, ILTIFa, IL22

Product Properties

Formulation Lyophilized from sterile PBS,pH 7.4.
Features & Benefits <0.1 EU per μg of the protein as determined by the LAL method.
Molecular weight 17.58 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.
Storage instructions Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA,10% FBS or 0.1%BSA). Please prevent freeze-thaw cycles.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Interleukin 22 (IL-22) is an α-helical cytokine, predicts a molecular mass of 16.9 kDa. It is produced by T-helper (Th)-17 cells, γδ T cells, NKT cells and newly described innate lymphoid cells (ILCs). Effects involve stimulation of cell survival, proliferation and synthesis of antimicrobials including S110, Reg3β, Reg3γ and defensins.
Gene ID 50929
Alternative interleukin 22, IL-TIF, Cytokine ZCYTO18, If2b1, ILTIFa, IL22
Accession Q9JJY9

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