| Product name | Mouse IL-1α protein |
| Sequence | Amino acid sequence derived from Mouse IL-1α protein (NP034684.2) (Ser 115-Ser 270) was expressed. |
| Activity | ① Immobilized Mouse IL-1α protein at 1 μg/mL (100 μl/well) can bind Mouse IL1R1, The EC50 of mouse IL1R1 is 1.562 μg/mL; ② Measured in a proliferation assay using CTLL-2 Mouse T lymphocytes cell. The ED50 for this effect is 1.64 pg/mL, corresponding to a specific activity is 6.098×108 units/mg. |
| Protein length | The recombinant Mouse IL-1α protein consists of 156 amino acids and has a predicted molecular mass of 18 kDa. |
| Preparation method | E. coli |
| Purity | >98% as determined by SDS-PAGE |
| Alternative | rMuIL-1α; Hematopoietin-1; IL1A; IL1F1; IL-1 alpha |
| Formulation | Lyophilized from sterile 20 mM Tris with 150 mM NaCl, pH 7.4. |
| Features & Benefits | Endotoxin:< 0.01 EU per μg of the protein as determined by the LAL method. |
| Molecular weight | 18 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse IL-1α protein using the buffer we provided not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage instructions | Lyophilized Mouse IL-1α protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | IL-1α is a member of the interleukin 1 cytokine family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation, and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. IL-1α is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. It is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. IL-1α stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. |
| Gene ID | 16175 |
| Alternative | rMuIL-1α; Hematopoietin-1; IL1A; IL1F1; IL-1 alpha |
Fig.SDS-PAGE analysis of Mouse IL-1α protein
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