| Product name | Mouse IFN-γ Protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Mouse IFN-γ protein (His23-Cys155)(P01580) was expressed with 6×His tag at the C-terminus |
| Applications | His tag |
| Applications notes | Mouse |
| Activity | Measure by its ability to anti-viral assay in L-929 cells infected with encephalomyocarditis (EMC) virus.The ED₅₀ for this effect is <0.5 ng/mL.The specific activity of recombinant mouse IFN gamma is approximately >2x 10⁶ IU/mg. |
| Protein length | The Mouse IFN-γ protein consists of 133 amino acids and has a predicted molecular mass of 16.5 kDa |
| Preparation method | E. coli |
| Purity | >98% as determined by SDS-PAGE. |
| Alternative | Type II interferon, T-cell interferon, MAF, Ifg, If2f, IFN-g |
| Formulation | Lyophilized from sterile PBS, pH 7.4 |
| Features & Benefits | Endotoxin:< 0.01 EU per μg of the protein as determined by the LAL method. |
| Molecular weight | The protein has a calculated MW of 16.5 kDa. The protein migrates as 16 kDa under reducing condition (SDS-PAGE analysis). |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please |
| Storage instructions | Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | IFN-γ is an acid-labile interferon produced by CD4 and CD8 T lymphocytes, as well as activated NK cells. IFN-γ receptors are present in most immune cells, which respond to IFN-γ signaling by increasing the surface expression of classⅠ MHC proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. IFN-γ signaling in antigen-presenting, cells and antigen-recognizing B and T lymphocytes, regulates the antigen-specific phases of the immune response |
| Alternative | Type II interferon, T-cell interferon, MAF, Ifg, If2f, IFN-g |
| Accession | P01580 |
Fig.SDS-PAGE analysis of Mouse IFN-γ
Author：余云健 Publication name：Applied genetics and molecular biotechnology IF：11.5
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