| Product name | Mouse HMGB1 Protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from human IL-2 isoform (P60568-1) (Ala21-Thr153) was expressed. |
| Applications | His tag |
| Applications notes | Mouse |
| Activity | Measured by its ability to induce TNF alpha in RAW264.7 cells. The ED50 for this effect is ＜15 ng/mL. |
| Protein length | The recombinant Mouse HMGB1 consists of 215 amino acids and predicts a molecular mass of 25.56 kDa. |
| Preparation method | E. coli |
| Purity | > 95% as determined by SDS-PAGE |
| Alternative | HMG-1, HMG1, HMG3, SBP-1 |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Features & Benefits | Endotoxin: < 0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight | 25.56 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of not less than 100 ug/mL using sterile ddHO, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously. |
| Storage instructions | Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles. |
| Shipping | The product is shipped at ambient temperature. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | HMGB1 is present in the nuclei (chromatin associated) and cytoplasm of all cells and is a highly conserved protein in variety of species that. In the cytoplasm, HMGB1 is a regulator of autophagy, enhances cell survival, and limits apoptosis. It also can reduces protein aggregation caused by heat or chemical stress. HMGB1 is released to the extracellular milieu by inflammatory cells and by necrotic and apoptotic cells. Once released, it works as an inflammatory cytokine. HMGB1 is also secreted by macrophages and monocytes as a late response to LPS, TNF-α, IL-1, or IFN-γ. |
| Alternative | HMG-1, HMG1, HMG3, SBP-1 |
| Accession | P63158 |
Fig.Mouse HMGB1 Protein, His tag (Animal-Free)
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