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Mouse EGF protein, His tag (Animal-Free)

Mouse EGF protein, His tag (Animal-Free)

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Specification

Product name Mouse EGF protein, His tag (Animal-Free)
Sequence Amino acid sequence derived from Mouse EGF (Met978-Arg 1029)(P01132) was expressed with 6×His tag at the C-terminus
Applications His tag
Applications notes Mouse
Activity Measure by its ability to induce 3T3 cells proliferation.The ED₅₀ for this effect is <80 pg/mL.The specific activity of recombinant mouse EGF is approximately >1 x 10⁷ IU/mg.
Protein length The protein has a calculated MW of 6.98 kDa.The protein migrates as 8 kDa under reducing condition (SDS-PAGE analysis).
Preparation method E. coli
Purity >98% as determined by SDS-PAGE.
Alternative Urogastrone, URG

Product Properties

Formulation The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us.
Features & Benefits Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method.
Molecular weight The protein has a calculated MW of 6.98 kDa.The protein migrates as 8 kDa under reducing condition (SDS-PAGE analysis).
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant Mouse EGF protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously
Storage instructions Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Epidermal Growth Factors (EGF) is a member of the EGF-family of proteins. EGF is mainly secreted from ectodermal cells, monocytes, kidney and duodenal glands. Upon binding to its receptor, EGFR, EGF acts to stimulate cell growth and proliferation of epithelial cells, play important roles in many developmental processes including accelerate tooth eruption, inhibits gastric acid secretion, and involve in wound healing.
Alternative Urogastrone, URG
Accession P01132

Image & description

Fig.SDS-PAGE analysis of Mouse EGF protein

Fig.SDS-PAGE analysis of Mouse EGF protein

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