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Mouse Ceramide glucosyltransferase (UGCG) ELISA Kit

Mouse Ceramide glucosyltransferase (UGCG) ELISA Kit

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Specification

Product name Mouse Ceramide glucosyltransferase (UGCG) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Ceramide glucosyltransferase (UGCG) ELISA Kit employs a two-site sandwich ELISA to quantitate UGCG in samples. An antibody specific for UGCG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUGCG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UGCG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UGCG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative UGCG; GCS; GLCT1; UDP-glucose:N-acylsphingosine D-glucosyltransferase; ceramide glucosyltransferase; glucosylceramide synthase

Product Properties

Kit components • Mouse Ceramide glucosyltransferase microplate
• Mouse Ceramide glucosyltransferase standard
• Mouse Ceramide glucosyltransferase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Ceramide glucosyltransferase (UGCG) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse UGCG. No significant cross-reactivity or interference between Mouse UGCG and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Glycosphingolipids (GSLs) are a group of membrane components that contain lipid and sugar moieties. They are present in essentially all animal cells and are believed to have important roles in various cellular processes. UDP-glucose ceramide glucosyltransferase (UGCG) catalyzes the first glycosylation step in GSL biosynthesis. The product, glucosylceramide, is the core structure of more than 300 GSLs. Ichikawa et al. cloned a UGCG cDNA by expressing a human melanoma cell cDNA library in a UGCG-deficient cell line and screening for UGCG complementation. The predicted 394-amino acid UGCG protein has a potential signal-anchor sequence and a single transmembrane domain near the N terminus, and very hydrophobic regions close to the C terminus, which may interact with the membrane.
Gene ID 22234
Alternative UGCG; GCS; GLCT1; UDP-glucose:N-acylsphingosine D-glucosyltransferase; ceramide glucosyltransferase; glucosylceramide synthase
Accession O88693

Image & description

Fig.1. Mouse Ceramide glucosyltransferase (UGCG) Standard Curve.

Fig.1. Mouse Ceramide glucosyltransferase (UGCG) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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