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Mouse BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2 (TNFAIP1) ELISA Kit

Mouse BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2 (TNFAIP1) ELISA Kit

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Specification

Product name Mouse BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2 (TNFAIP1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2 (TNFAIP1) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFAIP1 in samples. An antibody specific for TNFAIP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFAIP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFAIP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFAIP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TNFAIP1; B12; B61; EDP1; MGC2317; tumor necrosis factor; alpha-induced protein 1

Product Properties

Kit components • Mouse BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2 microplate
• Mouse BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2 standard
• Mouse BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2 (TNFAIP1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNFAIP1. No significant cross-reactivity or interference between Mouse TNFAIP1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background TNFAIP1 was identified as a gene whose expression can be induced by the tumor necrosis factor alpha (TNF) in umbilical vein endothelial cells. Studies of a similar gene in mouse suggest that the expression of this gene is developmentally regulated in a tissue-specific manner. Wolf et al. (1992) characterized a novel cDNA by differential screening of a tumor necrosis factor-alpha (TNFA)-stimulated umbilical vein endothelial cell library.The protein product is involved in the primary response of the endothelium to TNF. The 3.5-kb transcript was found to be expressed in a developmentally regulated, tissue-specific manner. The gene, present in single copy, was located in the 17q22-q23 region by in situ hybridization.
Gene ID 21927
Alternative TNFAIP1; B12; B61; EDP1; MGC2317; tumor necrosis factor; alpha-induced protein 1
Accession O70479

Image & description

Fig.1. Mouse BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2 (TNFAIP1) Standard Curve.

Fig.1. Mouse BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2 (TNFAIP1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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