Product name | Mouse bFGF protein |
Sequence | Amino acid sequence derived from Mouse bFGF soluble form (NP_15655) (Pro10-Ser154) was expressed, with an initial Met at the N-terminus. |
Activity | Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.2 ng/mL. |
Protein length | The recombinant Mouse bFGF protein consists of 149 amino acids and predicts a molecular mass of 16.4 kDa. |
Preparation method | E. coli |
Purity | > 95 % as determined by SDS-PAGE |
Alternative | BFGF; FGF-2; FGFB; HBGF-2; Fibroblast growth factor 2; FGF2 |
Formulation | Lyophilized from sterile 400mM NaCl 20mM PB , pH 7.0. |
Molecular weight | 16.4 kDa |
Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse bFGF protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Storage instructions | Lyophilized Mouse bFGF proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.4% HSA or BSA). Please prevent freeze-thaw cycles. |
Shipping | The product is shipped at ambient temperature. |
Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Background | Basic fibroblast growth factor (bFGF), also known as FGF2, is a member of the fibroblast growth factor (FGF) family. It is a highly specific chemotactic and mitogenic factor for many cell types, appears to be involved in remodeling damaged tissue, such as ulcer healing, vascular repair, traumatic brain injury (TBI). bFGF is a critical component of human embryonic stem cell culture medium. In addition, bFGF protein is a heparin-binding cationic protein involved in a variety of pathological conditions including angiogenesis and solid tumour growth. Thus, bFGF is regarded as a target for cancers chemopreventive and therapeutic strategies. |
Gene ID | 14173 |
Alternative | BFGF; FGF-2; FGFB; HBGF-2; Fibroblast growth factor 2; FGF2 |
Accession | P15655 |
Fig.SDS-PAGE analysis of Mouse bFGF protein.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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