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MMP-9 Polyclonal Antibody

MMP-9 Polyclonal Antibody

Views(596) Publications(1) Catalog no(ABP51421)
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Specification

Product name MMP-9 Polyclonal Antibody
Immunogen Synthesized peptide derived from the C-terminal region of human MMP-9 at AA range: 620-700
Host Rabbit
Reactivity Human
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative MMP9; CLG4B; Matrix metalloproteinase-9; MMP-9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. Matrix metalloproteinase-9 encoded by MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
Gene ID 4318
Alternative MMP9; CLG4B; Matrix metalloproteinase-9; MMP-9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB
Others MMP-9 Polyclonal Antibody detects endogenous levels of MMP-9 protein.
Accession P14780
Observed Band(KD) 78

Image & description

Fig.1. Western Blot analysis of various cells using MMP-9 Polyclonal Antibody diluted at 1:1000.

Fig.1. Western Blot analysis of various cells using MMP-9 Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of HepG2 cells using MMP-9 Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of HepG2 cells using MMP-9 Polyclonal Antibody diluted at 1:1000.

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