| Product name | Mitochondrial Superoxide Assay Kit with MitoNeoD |
| Applications notes | MitoNeoD (also known as MND, Mito-NeoD, Mito Neo D, Mito Neo-D) is a fluorescent probe that can rapidly and specifically target mitochondria and selectively detect mitochondrial O₂•⁻. Its structure consists of three functional units: the reduced phthalazine core serves as an O₂•⁻ responsive group; when not oxidized, the fluorescence is extremely weak, and upon oxidation, a red fluorescent product is generated; the new octyl substitution can spatially prevent the product from embedding into DNA; the 6-carbon-deuterium bond undergoes kinetic isotope effect (KIE) to reduce the non-specific oxidation rate and improve the selectivity for O₂•⁻; the triphenylphosphonium lipophilic cation, driven by membrane potential, crosses the cell membrane and accumulates in the mitochondrial matrix to achieve mitochondrial targeting. |
| SampleType | Adherent cells, suspension cells |
| Kit components | •MitoNeoD（5 mM） •Assay Buffer •mSox UP |
| Features & Benefits | 1. Specific upgrade: Precisely target mitochondrial superoxide anion, effectively eliminating irrelevant signal interference. 2. Companion positive control: Comes with mSox UP positive induction reagent, facilitating the construction of positive models and ensuring controllable experimental results. 3. Compatible with multiple scenarios: Can be detected for both adherent and suspension cells; compatible with mainstream fluorescence equipment such as fluorescence microscopes, flow cytometers, and fluorescence microplate readers. |
| Storage instructions | Stored at -20℃ for 6 months, protected from light |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | MitoNeoD (also known as MND, Mito-NeoD, Mito Neo D, Mito Neo-D) is a fluorescent probe that can rapidly and specifically target mitochondria and selectively detect mitochondrial O₂•⁻. Its structure consists of three functional units: the reduced phthalazine core serves as an O₂•⁻ responsive group; when not oxidized, the fluorescence is extremely weak, and upon oxidation, a red fluorescent product is generated; the new octyl substitution can spatially prevent the product from embedding into DNA; the 6-carbon-deuterium bond undergoes kinetic isotope effect (KIE) to reduce the non-specific oxidation rate and improve the selectivity for O₂•⁻; the triphenylphosphonium lipophilic cation, driven by membrane potential, crosses the cell membrane and accumulates in the mitochondrial matrix to achieve mitochondrial targeting. |
Fig.Mitochondrial Superoxide Assay Kit with MitoNeoD.
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