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MEF-2D (phospho Ser444) Polyclonal Antibody

MEF-2D (phospho Ser444) Polyclonal Antibody

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Specification

Product name MEF-2D (phospho Ser444) Polyclonal Antibody
Immunogen Synthesized peptide derived from human MEF-2D around the phosphorylation site of S444
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative MEF2D; Myocyte-specific enhancer factor 2D

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MEF2D is a member of the myocyte-specific enhancer factor 2 (MEF2) family of transcription factors. Members of this family are involved in control of muscle and neuronal cell differentiation and development, and are regulated by class II histone deacetylases. Fusions of the encoded protein with Deleted in Azoospermia-Associated Protein 1 (DAZAP1) due to a translocation have been found in an acute lymphoblastic leukemia cell line, suggesting a role in leukemogenesis. The encoded protein may also be involved in Parkinson disease and myotonic dystrophy. Alternative splicing results in multiple transcript variants.
Gene ID 4209
Alternative MEF2D; Myocyte-specific enhancer factor 2D
Others Phospho-MEF-2D (S444) Polyclonal Antibody detects endogenous levels of MEF-2D protein only when phosphorylated at S444.
Accession Q14814
Observed Band(KD) 55

Image & description

Fig. Western blot analysis of K562 AD293 using Phospho-MEF-2D (S444) antibody. Antibody was diluted at 1:500.

Fig. Western blot analysis of K562 AD293 using Phospho-MEF-2D (S444) antibody. Antibody was diluted at 1:500.

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