| Product name | M-CSF Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the C-terminal region of human M-CSF |
| Host | Rabbit |
| Reactivity | Human,Rat,Mouse, |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC: 1:100-1:300;ELISA 1:10000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | CSF1; Macrophage colony-stimulating factor 1; CSF-1; M-CSF; MCSF; Lanimostim |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 48kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Macrophage colony-stimulating factor 1 encoded by CSF1 is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. |
| Gene ID | 1435 |
| Alternative | CSF1; Macrophage colony-stimulating factor 1; CSF-1; M-CSF; MCSF; Lanimostim |
| Others | M-CSF Polyclonal Antibody detects endogenous levels of M-CSF protein. |
| Accession | P09603 |
| Observed Band(KD) | 48 |
Fig.1. Western Blot analysis of NIH-3T3, hela cells using M-CSF Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Western Blot analysis of 3T3 cells using M-CSF Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.3. Immunohistochemical analysis of paraffin-embedded human-tonsils, antibody was diluted at 1:100.
Fig.4. Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100.
Fig.5. Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100.
Fig.6. Western blot analysis of various cell Lysate, antibody was diluted at 1:1000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
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