| Product name | Luciferase firefly |
| Sequence | AminoacidsequenceofluciferasederivedfromPhotinuspyralis(firefly)wasexpressed. |
| Activity | >109 RLU/mg protein as determined in the presence of ATP and luciferin. |
| Protein length | The recombinant Firefly Luciferase predicts a molecular mass of 62 kDa. |
| Preparation method | E. coli |
| Purity | > 98 % as determined by SDS-PAGE |
| Formulation | Buffered aqueous solution contains 20mM Tris, 150mM NaCl, 1mM EDTA, 15% glycerol, pH 8.0 |
| Molecular weight | 62 kDa |
| Usage notes | Always centrifuge tubes before opening. |
| Storage instructions | The recombinant Luciferase Firefly product should be protected from light and stored below -20°C. To keep the enzyme activity and for long term storage, it is recommended to be aliquoted and prevent freeze-thaw cycles. |
| Shipping | The product is shipped by ice pack or gel pack with blue ice |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Firefly Luciferase catalyzes the reaction of luciferin with ATP and results in the production of yellow-green light. The enzyme has a molecular weight of 62 kDa and is expressed in peroxisomes. Luciferase is considered a model for studying protein-anesthetic interactions. Firefly Luciferase is very useful in cell biology and molecular biology as a reporter of gene function and for the quantification of ATP |
Fig. SDS-PAGE analysis of Luciferase Firefly
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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