- Product name
Live Cell Tracking Kit (Green Fluorescence)
- Application notes
Abbkine Live Cell Tracking Kit (Green Fluorescence) provides a versatile and well-retained celltracing reagent (CellTracker Green) for monitoring cell movement, location, proliferation, migration, chemotaxis, and invasion. CellTracker Green can passively diffuses into cells and remain colorless and nonfluorescent until its acetate groups are cleaved by intracellular esterases to yield highly fluorescent, amine-reactive carboxyfluorescein succinimidyl ester. The succinimidyl ester group reacts with intracellular amines, forming fluorescent conjugates (Ex/Em= 494/521nm) that are well-retained and can be fixed with aldehyde fixatives.
- Kit components
• CellTracker Green
• Assay Buffer (5×)
- Features & Benefits
• Useful for monitoring cell movement, location, proliferation, migration, chemotaxis, and invasion.
• The CellTracker Green probes are well retained in living cells through several generations and can display fluorescence for at least a week. The probes are transferred to daughter cells, but are not transferred to adjacent cells in a population.
• Optimized for flow cytometery or fluorescent microscopy.
- Usage notes
Thaw all the components at room temperature before opening
- Storage instructions
Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment.
Gel pack with blue ice.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Cell movement and location studies require specialized probes that are nontoxic to living cells. Commercial Live Cell Tracking Kits provide a convenient method for monitoring cell movement, location, proliferation, migration, chemotaxis, and invasion.
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Live Cell Tracking Kit (Green Fluorescence)
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Notice:Protocol may vary from different lot which is subject to the manual along with the shipment. Please contact firstname.lastname@example.org for your enquiry.
Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.
Preparation methods for Biochemical
Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.
Antibody application protocols
Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.
- ♦ Antibody Western Blotting (WB) protocol
- ♦ Antibody Immunohistochemistry (IHC) protocol
- ♦ Antibody Immunofluorescence (IF) protocol
- ♦ Antibody Immunoprecipitation (IP) protocol
- ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol
Protein&peptide usage suggestions
Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.
- ♦ Handling and storage suggestion for peptides and protein
- ♦ Cytokines and growth factors for cell culture application
Commonly used assay kits guidelines
Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.
We hope this will be helpful for your research work. Please let us know through email@example.com if you need more information or support.