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LIMK-1 (phospho Thr508) Polyclonal Antibody

LIMK-1 (phospho Thr508) Polyclonal Antibody

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Specification

Product name LIMK-1 (phospho Thr508) Polyclonal Antibody
Immunogen Synthesized peptide derived from human LIMK-1 around the phosphorylation site of T508
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative LIMK1; LIMK; LIM domain kinase 1; LIMK-1

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. LIM domain kinase 1 is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. LIM domain kinase 1 also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Gene ID 3984
Alternative LIMK1; LIMK; LIM domain kinase 1; LIMK-1
Others Phospho-LIMK-1 (T508) Polyclonal Antibody detects endogenous levels of LIMK-1 protein only when phosphorylated at T508.
Accession P53667
Observed Band(KD) 72

Image & description

Fig.1. Western Blot analysis of various cells using Phospho-LIMK-1 (T508) Polyclonal Antibody diluted at 1:1000.

Fig.1. Western Blot analysis of various cells using Phospho-LIMK-1 (T508) Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of 293 cells using Phospho-LIMK-1 (T508) Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of 293 cells using Phospho-LIMK-1 (T508) Polyclonal Antibody diluted at 1:1000.

Fig.3. Western Blot analysis of HEPG2 using Phospho-LIMK-1 (T508) Polyclonal Antibody. Antibody was diluted at 1:1000.

Fig.3. Western Blot analysis of HEPG2 using Phospho-LIMK-1 (T508) Polyclonal Antibody. Antibody was diluted at 1:1000.

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