- Product name
IPKine™ HRP, Mouse Anti-Rabbit IgG LCS
Rabbit IgG light chain
ELISA, IHC, WB
- Application notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:10000-1:100000), IHC (1:500-1:5000), ELISA (1:5000-1:100000).
Affinity purified using solid phase Rabbit IgG(H+L) light chain with finally > 95% purity based on SDS-PAGE
- Features & Benefits
50kD or 25kD protein detection on Western blots after Immunoprecipitation is often suffered from heavy or light chain blotting contamination). Abbkine's IPKine™ products solve these problems through less background noise, and enhanced accuracy). Their light chain/Fc fragment specific optimization will eliminate heavy/light chain interference, while serum proteins absorbed minimize cross-reactivity with immunoglobulins from other species which may be present on blots).
- Storage buffer
Liquid in PBS, pH 7.4, containing 1% BSA as stablizer and 50% Glycerol.
- Storage instructions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Gel pack with blue ice.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application.
The antibody reacts with kappa light chains on rabbit IgG, while it doesn’t react with the heavy chain of rabbit IgG. It's cross-reaction with human, mouse, rat, goat, sheep and bovine serum proteins has been specialized minimized, while it may cross-react with immunoglobulins from other species. It has no reactivity on non-immunoglobulin serum proteins
Most popular with customers
Application: FCM, ICC, IF
Application: ELISA, IHC, WB
Application: FCM, ICC, IF
Application: FCM, ICC, IF
Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.
Preparation methods for Biochemical
Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.
Antibody application protocols
Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.
- ♦ Antibody Western Blotting (WB) protocol
- ♦ Antibody Immunohistochemistry (IHC) protocol
- ♦ Antibody Immunofluorescence (IF) protocol
- ♦ Antibody Immunoprecipitation (IP) protocol
- ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol
Protein&peptide usage suggestions
Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.
- ♦ Handling and storage suggestion for peptides and protein
- ♦ Cytokines and growth factors for cell culture application
Commonly used assay kits guidelines
Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.
We hope this will be helpful for your research work. Please let us know through email@example.com if you need more information or support.
Ets-1 deficiency alleviates nonalcoholic steatohepatitis via weakening TGF-β1 signaling-mediated hepatocyte apoptosis
Dechen Liu, Kai Wang, Kai Li, et al. Cell Death Dis. 2019 Jun 12;10(6):458.
SIRT2 Inhibition Results in Meiotic Arrest, Mitochondrial Dysfunction, and Disturbance of Redox Homeostasis during Bovine Oocyte Maturation
Dejun Xu, Lin Wu, Xiaohan Jiang, et al. Int J Mol Sci. 2019 Mar 18;20(6).
Acinetobacter baumannii outer membrane protein A induces HeLa cell autophagy via MAPK/JNK signaling pathway
Zhiyuan An, Xiaoxi Huang, Chunming Zheng and Wenyi Ding. Int J Med Microbiol. 2019 Mar;309(2):97-107.
Positive-Feedback Regulation of Subchondral H-Type Vessel Formation by Chondrocyte Promotes Osteoarthritis Development in Mice
Lu J, Zhang H, Cai D, et al. J Bone Miner Res, 2018.
Disruption of Ssp411 causes impaired sperm head formation and male sterility in mice
Liu M, Ru Y, Gu Y, et al. Biochim Biophys Acta, 2018, 1862(3): 660-668.
Hepatitis B X-interacting protein promotes the formation of the insulin gene–transcribing protein complex Pdx-1/Neurod1 in animal pancreatic β-cells
Li H, Wang Z, Li Y H, et al. JOURNAL OF BIOLOGICAL CHEMISTRY, 2017, 293: 2053-2065.
Bre Enhances Osteoblastic Differentiation by Promoting the Mdm2-Mediated Degradation of p53
Jin, Fujun, et al. STEM CELLS (2017).