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IL-6 Polyclonal Antibody

IL-6 Polyclonal Antibody

Views(1061) Publications(0) Catalog no(ABP0137)
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Specification

Product name IL-6 Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human IL-6
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, ICC, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB(1:500-1:2000),IHC-P(1:100-1:300),ELISA(1:20000),IF(1:100-1:300).
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative IL6; IFNB2; Interleukin-6; IL-6; B-cell stimulatory factor 2; BSF-2; CTL differentiation factor; CDF; Hybridoma growth factor; Interferon beta-2; IFN-beta-2

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 23 KD
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background IL6 encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the interleukin 6 has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants.
Gene ID 3569
Alternative IL6; IFNB2; Interleukin-6; IL-6; B-cell stimulatory factor 2; BSF-2; CTL differentiation factor; CDF; Hybridoma growth factor; Interferon beta-2; IFN-beta-2
Others IL-6 Polyclonal Antibody detects endogenous levels of IL-6 protein.
Accession P05231

Image & description

Fig.1. Western Blot analysis of K562 cells using IL-6 Polyclonal Antibody. Antibody was diluted at 1:1000.

Fig.1. Western Blot analysis of K562 cells using IL-6 Polyclonal Antibody. Antibody was diluted at 1:1000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human breast cancer tissue. 1, IL-6 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.2. Immunohistochemical analysis of paraffin-embedded human breast cancer tissue. 1, IL-6 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

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