| Product name | IFKine™ Orange Donkey Anti-Mouse IgG |
| Immunogen | Mouse IgG whole molecule |
| Host | Donkey |
| Reactivity | Mouse |
| Applications | FCM, ICC, IF |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting 1:50-1:1000 dilutions for most fluorescent applications. |
| Clonality | Polyclonal |
| Preparation method | Affinity purified using solid phase Mouse IgG (H&L) with finally > 95% purity based on SDS-PAGE |
| Formulation | Liquid solution |
| Features & Benefits | Abbkine IFKine™ is series of unique fluorence staining secondary antibodies with improved brightness, photostability and less nonspecific hybridization and background). The latest generation of IFKine fluorescent dyes ensure the best fluorescent performance, while it’s donkey host and other species of serum/IgG absorbed make IFKine™ secondary antibodies the ideal for use in fluorence staining, especially in fluorence multiple labeling). |
| Storage buffer | Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative, 1% BSA as stablizer and 50% Glycerol. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. |
| Others | The antibody reacts with whole molecule mouse IgG. It also reacts with light chains of all other mouse immunoglobulins. It's cross-reaction with human, rabbit, goat, sheep and guinea pig serum proteins has been specialized minimized to reduce non-specific hybrization. It has no reactivity on non-immunoglobulin serum proteins |
Author：J Huang, H Li, Z Yang, R Liu Publication name：cell death & disease IF：9
Author：Meng S, Wang Z, Liu X Publication name：Cellular Signalling IF：4.80
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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