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HXK I Polyclonal Antibody

HXK I Polyclonal Antibody

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Specification

Product name HXK I Polyclonal Antibody
Immunogen Synthesized peptide derived from the N-terminal region of human HXK I at AA range: 1-80
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative HK1; Hexokinase-1; Brain form hexokinase; Hexokinase type I; HK I

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. HK1 encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in HK1 have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific.
Gene ID 3098
Alternative HK1; Hexokinase-1; Brain form hexokinase; Hexokinase type I; HK I
Others HXK I Polyclonal Antibody detects endogenous levels of HXK I protein.
Accession P19367
Observed Band(KD) 109

Image & description

Fig.1. Western Blot analysis of various cells using HXK I Polyclonal Antibody diluted at 1:1000.

Fig.1. Western Blot analysis of various cells using HXK I Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of hela cells using HXK I Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of hela cells using HXK I Polyclonal Antibody diluted at 1:1000.

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