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Human WNT1-inducible-signaling pathway protein 1 (WISP1) ELISA Kit

Human WNT1-inducible-signaling pathway protein 1 (WISP1) ELISA Kit

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Specification

Product name Human WNT1-inducible-signaling pathway protein 1 (WISP1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human WNT1-inducible-signaling pathway protein 1 (WISP1) ELISA Kit employs a two-site sandwich ELISA to quantitate WISP1 in samples. An antibody specific for WISP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWISP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WISP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WISP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative WISP1; CCN4; WISP1c; WISP1i; WISP1tc; WNT1 induced secreted protein 1; Wnt-1 inducible signaling pathway protein 1; wnt-1 signaling pathway protein 1

Product Properties

Kit components • Human WNT1-inducible-signaling pathway protein 1 microplate
• Human WNT1-inducible-signaling pathway protein 1 standard
• Human WNT1-inducible-signaling pathway protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human WNT1-inducible-signaling pathway protein 1 (WISP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human WISP1. No significant cross-reactivity or interference between Human WISP1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background WISP1 encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Alternative splicing of this gene generates 2 transcript variants.
Gene ID 8840
Alternative WISP1; CCN4; WISP1c; WISP1i; WISP1tc; WNT1 induced secreted protein 1; Wnt-1 inducible signaling pathway protein 1; wnt-1 signaling pathway protein 1
Accession O95388

Image & description

Fig.1. Human WNT1-inducible-signaling pathway protein 1 (WISP1) Standard Curve.

Fig.1. Human WNT1-inducible-signaling pathway protein 1 (WISP1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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