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Human VPS10 domain-containing receptor SorCS3 (SORCS3) ELISA Kit

Human VPS10 domain-containing receptor SorCS3 (SORCS3) ELISA Kit

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Specification

Product name Human VPS10 domain-containing receptor SorCS3 (SORCS3) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human VPS10 domain-containing receptor SorCS3 (SORCS3) ELISA Kit employs a two-site sandwich ELISA to quantitate SORCS3 in samples. An antibody specific for SORCS3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySORCS3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SORCS3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SORCS3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SORCS3; RP11-107L7.1; SORCS; 6330404A12Rik; VPS10 domain receptor protein SORCS 3; VPS10 domain receptor protein SORCS 3 (SORCS3)

Product Properties

Kit components • Human VPS10 domain-containing receptor SorCS3 microplate
• Human VPS10 domain-containing receptor SorCS3 standard
• Human VPS10 domain-containing receptor SorCS3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human VPS10 domain-containing receptor SorCS3 (SORCS3) ELISA Kit has high sensitivity and excellent specificity for detection of Human SORCS3. No significant cross-reactivity or interference between Human SORCS3 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background SORCS3 encodes a type-I receptor transmembrane protein that is a member of the vacuolar protein sorting 10 receptor family. Proteins of this family are defined by a vacuolar protein sorting 10 domain at the N-terminus. The N-terminal segment of this domain has a consensus motif for proprotein convertase processing, and the C-terminal segment of this domain is characterized by ten conserved cysteine residues. The vacuolar protein sorting 10 domain is followed by a leucine-rich segment, a transmembrane domain, and a short C-terminal cytoplasmic domain that interacts with adaptor molecules. The transcript is expressed at high levels in the brain, and candidate gene studies suggest that genetic variation in this gene is associated with Alzheimer's disease. Consistent with this observation, knockdown of SORCS3 in cell culture results in an increase in amyloid precursor protein processing.
Gene ID 22986
Alternative SORCS3; RP11-107L7.1; SORCS; 6330404A12Rik; VPS10 domain receptor protein SORCS 3; VPS10 domain receptor protein SORCS 3 (SORCS3)
Accession Q9UPU3

Image & description

Fig.1. Human VPS10 domain-containing receptor SorCS3 (SORCS3) Standard Curve.

Fig.1. Human VPS10 domain-containing receptor SorCS3 (SORCS3) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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