Login Register
English
0

Cart

$ 0

Human Vitamin K epoxide reductase complex subunit 1 (VKORC1) ELISA Kit

Human Vitamin K epoxide reductase complex subunit 1 (VKORC1) ELISA Kit

Views(96) Publications(0) Catalog no(KTE60051)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Human Vitamin K epoxide reductase complex subunit 1 (VKORC1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Vitamin K epoxide reductase complex subunit 1 (VKORC1) ELISA Kit employs a two-site sandwich ELISA to quantitate VKORC1 in samples. An antibody specific for VKORC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVKORC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VKORC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VKORC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative VKORC1; EDTP308; FLJ00289; IMAGE3455200; MGC2694; MST134; MST576; VKCFD2; VKOR; phylloquinone epoxide reductase; vitamin K dependent clotting factors deficiency 2; vitamin K1 epoxide reductase (warfarin-sensi

Product Properties

Kit components • Human Vitamin K epoxide reductase complex subunit 1 microplate
• Human Vitamin K epoxide reductase complex subunit 1 standard
• Human Vitamin K epoxide reductase complex subunit 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Vitamin K epoxide reductase complex subunit 1 (VKORC1) ELISA Kit has high sensitivity and excellent specificity for detection of Human VKORC1. No significant cross-reactivity or interference between Human VKORC1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Vitamin K is essential for blood clotting but must be enzymatically activated. This enzymatically activated form of vitamin K is a reduced form required for the carboxylation of glutamic acid residues in some blood-clotting proteins. VKORC1 is responsible for reducing vitamin K 2,3-epoxide to the enzymatically activated form. Fatal bleeding can be caused by vitamin K deficiency and by the vitamin K antagonist warfarin, and it is the product of this gene that is sensitive to warfarin. In humans, mutations in this gene can be associated with deficiencies in vitamin-K-dependent clotting factors and, in humans and rats, with warfarin resistance. Two pseudogenes have been identified on chromosome 1 and the X chromosome. Two alternatively spliced transcripts encoding different isoforms have been described.
Gene ID 79001
Alternative VKORC1; EDTP308; FLJ00289; IMAGE3455200; MGC2694; MST134; MST576; VKCFD2; VKOR; phylloquinone epoxide reductase; vitamin K dependent clotting factors deficiency 2; vitamin K1 epoxide reductase (warfarin-sensi
Accession Q9BQB6

Image & description

Fig.1. Human Vitamin K epoxide reductase complex subunit 1 (VKORC1) Standard Curve.

Fig.1. Human Vitamin K epoxide reductase complex subunit 1 (VKORC1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Human Vitamin K epoxide reductase complex subunit 1 (VKORC1) ELISA Kit”