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Human Versican/PG-M/PG-350 (VS) ELISA Kit

Human Versican/PG-M/PG-350 (VS) ELISA Kit

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Specification

Product name Human Versican/PG-M/PG-350 (VS) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Versican/PG-M/PG-350 (VS) ELISA Kit employs a two-site sandwich ELISA to quantitate VCAN in samples. An antibody specific for VCAN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVCAN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VCAN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VCAN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative VCAN; CSPG2; VCAN; ERVR; PG-M; WGN; WGN1; Chondroitin Sulfate Proteoglycan 2; Versican Proteoglycan; DKFZp686K06110; GHAP; Glial hyaluronate-binding protein

Product Properties

Kit components • Human Versican/PG-M/PG-350 microplate
• Human Versican/PG-M/PG-350 standard
• Human Versican/PG-M/PG-350 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Versican/PG-M/PG-350 (VS) ELISA Kit has high sensitivity and excellent specificity for detection of Human VCAN. No significant cross-reactivity or interference between Human VCAN and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Versican is a large chondroitin sulfate proteoglycan with an apparent molecular mass of more than 1000kDa. In 1989, Zimmermann and Ruoslahti cloned and sequenced the core protein of fibroblast chondroitin sulfate proteoglycan. They designated it versican in recognition of its versatile modular structure. Versican belongs to the lectican protein family, with Aggrecan (abundant in Cartilage), Brevican and Neurocan (nervous system proteoglycans) as other members. Versican is also known as Chondroitin sulfate proteoglycan core protein 2 (CSPG-2), PG-M, and Chondroitin sulfate proteoglycan 2.These proteoglycans share a homologous globular N-terminal, C-terminal, and glycosaminoglycan (GAG) binding regions.
Gene ID 1462
Alternative VCAN; CSPG2; VCAN; ERVR; PG-M; WGN; WGN1; Chondroitin Sulfate Proteoglycan 2; Versican Proteoglycan; DKFZp686K06110; GHAP; Glial hyaluronate-binding protein
Accession P13611

Image & description

Fig.1. Human Versican/PG-M/PG-350 (VS) Standard Curve.

Fig.1. Human Versican/PG-M/PG-350 (VS) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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