|Product name||Human Vasodilator-stimulated phosphoprotein (VASP) ELISA Kit|
|Applications notes||This Human Vasodilator-stimulated phosphoprotein (VASP) ELISA Kit employs a two-site sandwich ELISA to quantitate VASP in samples. An antibody specific for VASP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVASP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VASP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VASP bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Human Vasodilator-stimulated phosphoprotein microplate
• Human Vasodilator-stimulated phosphoprotein standard
• Human Vasodilator-stimulated phosphoprotein detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Human Vasodilator-stimulated phosphoprotein (VASP) ELISA Kit has high sensitivity and excellent specificity for detection of Human VASP. No significant cross-reactivity or interference between Human VASP and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Synaptic vesicles are responsible for regulating the storage and release of neurotransmitters in the nerve terminal. Northern blot analysis revealed that a 5.8-kb transcript is expressed in electromotor neurons. Western blot analysis determined expression of a 42-kD protein in the electric organ that copurified with synaptic vesicles.Smith et al. (1996) identified the sequence of 2 complete genes within 117 kb of DNA containing the BRCA1 gene: RHO7 and VAT1, an abundant membrane protein of cholinergic synaptic vesicles. The coding sequence of VAT1 predicts a 301-amino acid peptide. The authors found that a CpG island precedes the VAT1 gene, which contains 6 exons spanning 8.1 kb. They determined the following order of genes in this region: cen--IFP35--VAT1--RHO7--BRCA1--1A1-3B--tel.|
Fig.1. Human Vasodilator-stimulated phosphoprotein (VASP) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.