Login Register
English
0

Cart

$ 0

Human Vasoactive intestinal polypeptide receptor 1 (VIPR1) ELISA Kit

Human Vasoactive intestinal polypeptide receptor 1 (VIPR1) ELISA Kit

Views(112) Publications(0) Catalog no(KTE60053)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Human Vasoactive intestinal polypeptide receptor 1 (VIPR1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Vasoactive intestinal polypeptide receptor 1 (VIPR1) ELISA Kit employs a two-site sandwich ELISA to quantitate VIPR1 in samples. An antibody specific for VIPR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVIPR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VIPR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VIPR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative VIPR1; FLJ41949; HVR1;; PACAP-R-2; RDC1; V1RG; VAPC1; VIPR; VIRG; VPAC1; VPCAP1R; PACAP type receptor; VIP receptor; type I; pituitary adenylate cyclase activating polypeptide receptor; type

Product Properties

Kit components • Human Vasoactive intestinal polypeptide receptor 1 microplate
• Human Vasoactive intestinal polypeptide receptor 1 standard
• Human Vasoactive intestinal polypeptide receptor 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Vasoactive intestinal polypeptide receptor 1 (VIPR1) ELISA Kit has high sensitivity and excellent specificity for detection of Human VIPR1. No significant cross-reactivity or interference between Human VIPR1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Vasoactive intestinal peptide receptor 1 is expressed in the brain (cerebral cortex, hippocampus, amygdala), lung, prostate, peripheral blood leukocytes, liver, small intestine, heart, spleen, placenta, kidney, thymus and testis . This gene encodes a receptor for vasoactive intestinal peptide, a small neuropeptide. Vasoactive intestinal peptide is involved in smooth muscle relaxation, exocrine and endocrine secretion, and water and ion flux in lung and intestinal epithelia. Its actions are effected through integral membrane receptors associated with a guanine nucleotide binding protein which activates adenylate
Gene ID 7433
Alternative VIPR1; FLJ41949; HVR1;; PACAP-R-2; RDC1; V1RG; VAPC1; VIPR; VIRG; VPAC1; VPCAP1R; PACAP type receptor; VIP receptor; type I; pituitary adenylate cyclase activating polypeptide receptor; type
Accession P32241

Image & description

Fig.1. Human Vasoactive intestinal polypeptide receptor 1 (VIPR1) Standard Curve.

Fig.1. Human Vasoactive intestinal polypeptide receptor 1 (VIPR1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Human Vasoactive intestinal polypeptide receptor 1 (VIPR1) ELISA Kit”