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Human V-rel avian reticuloendotheliosis viral oncogene related B (RELB) ELISA Kit

Human V-rel avian reticuloendotheliosis viral oncogene related B (RELB) ELISA Kit

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Specification

Product name Human V-rel avian reticuloendotheliosis viral oncogene related B (RELB) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human V-rel avian reticuloendotheliosis viral oncogene related B (RELB) ELISA Kit employs a two-site sandwich ELISA to quantitate RELB in samples. An antibody specific for RELB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRELB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RELB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RELB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative RELB; I-REL; IREL; REL-B; reticuloendotheliosis viral oncogene homolog B; v-rel avian reticuloendotheliosis viral oncogene homolog B (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3); v

Product Properties

Kit components • Human V-rel avian reticuloendotheliosis viral oncogene related B microplate
• Human V-rel avian reticuloendotheliosis viral oncogene related B standard
• Human V-rel avian reticuloendotheliosis viral oncogene related B detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human V-rel avian reticuloendotheliosis viral oncogene related B (RELB) ELISA Kit has high sensitivity and excellent specificity for detection of Human RELB. No significant cross-reactivity or interference between Human RELB and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Bours et al. (1994) demonstrated that human RELB, when generated in mammalian cells, formed kappa-B-binding heterodimeric complexes with p50 (NFKB1) or p52 (NFKB2). Homodimeric complexes of RELB did not show DNA-binding activity.NF-kappa-B-inducing kinase (NIK, or MAP3K14) is required for osteoclastogenesis in response to pathologic stimuli. Vaira et al. (2008) found that overexpression of Relb, but not Rela, rescued differentiation of mouse Nik -/- osteoclast precursors, indicating that blockade of the alternative NF-kappa-B pathway, rather than the classical NF-kappa-B pathway, is responsible for the osteoclastogenic defect in the absence of Nik. Using Relb -/- mice, they showed that Relb itself was required for Rankl-induced osteoclastogenesis in vitro and for TNF-induced bone resorption in vivo
Alternative RELB; I-REL; IREL; REL-B; reticuloendotheliosis viral oncogene homolog B; v-rel avian reticuloendotheliosis viral oncogene homolog B (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3); v

Image & description

Fig.1. Human V-rel avian reticuloendotheliosis viral oncogene related B (RELB) Standard Curve.

Fig.1. Human V-rel avian reticuloendotheliosis viral oncogene related B (RELB) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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