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Human Uracil-DNA glycosylase (UNG) ELISA Kit

Human Uracil-DNA glycosylase (UNG) ELISA Kit

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Specification

Product name Human Uracil-DNA glycosylase (UNG) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Uracil-DNA glycosylase (UNG) ELISA Kit employs a two-site sandwich ELISA to quantitate UNG in samples. An antibody specific for UNG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUNG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UNG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UNG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative UNG; DGU; DKFZp781L1143; HIGM4; UDG; UNG1; UNG15; UNG2; uracil-DNA glycosylase 1; uracil-DNA glycosylase 2; uracil-DNA glycosylase 2

Product Properties

Kit components • Human Uracil-DNA glycosylase microplate
• Human Uracil-DNA glycosylase standard
• Human Uracil-DNA glycosylase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Uracil-DNA glycosylase (UNG) ELISA Kit has high sensitivity and excellent specificity for detection of Human UNG. No significant cross-reactivity or interference between Human UNG and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background UNG encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. Alternative promoter usage and splicing of this gene leads to two different isoforms: the nuclear (UNG2) and mitochondrial (UNG1) isoforms of UNG result from alternative splicing and the use of alternative promoters. The UNG1 and UNG2 proteins contain different N-terminal sequences, but the downstream 269 amino acids are common and include a short region that binds replication protein A and a larger, compact catalytic domain.
Gene ID 7374
Alternative UNG; DGU; DKFZp781L1143; HIGM4; UDG; UNG1; UNG15; UNG2; uracil-DNA glycosylase 1; uracil-DNA glycosylase 2; uracil-DNA glycosylase 2
Accession P13051

Image & description

Fig.1. Human Uracil-DNA glycosylase (UNG) Standard Curve.

Fig.1. Human Uracil-DNA glycosylase (UNG) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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