| Product name | Human Ubiquitin-protein ligase E3A (UBE3A) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human Ubiquitin-protein ligase E3A (UBE3A) ELISA Kit employs a two-site sandwich ELISA to quantitate UBE3A in samples. An antibody specific for UBE3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUBE3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBE3A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBE3A bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | UBE3A; ANCR; AS; E6-AP; EPVE6AP; FLJ26981; HPVE6A; CTCL tumor antigen se37-2; human papilloma virus E6-associated protein; oncogenic protein-associated protein E6-AP |
| Kit components | • Human Ubiquitin-protein ligase E3A microplate • Human Ubiquitin-protein ligase E3A standard • Human Ubiquitin-protein ligase E3A detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human Ubiquitin-protein ligase E3A (UBE3A) ELISA Kit has high sensitivity and excellent specificity for detection of Human UBE3A. No significant cross-reactivity or interference between Human UBE3A and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Ubiquitin-protein ligase E3A (UBE3A) is an enzyme that in humans is encoded by the UBE3A gene. This enzyme is involved in targeting proteins for degradation within cells. Protein degradation is a normal process that removes damaged or unnecessary proteins and helps maintain the normal functions of cells. Ubiquitin protein ligase 3A attaches a small protein called ubiquitin to proteins that should be degraded. Cellular structures called proteasomes recognize and digest proteins tagged with ubiquitin. Both copies of the UBE3A gene are active in most of the body's tissues. In the brain, however, only the copy inherited from a person's mother (the maternal copy) is normally active. The UBE3A gene is located on the long (q) arm of chromosome 15 between positions 11 and 13, from base pair 23,133,488 to base pair 23,235,220. |
| Gene ID | 7337 |
| Alternative | UBE3A; ANCR; AS; E6-AP; EPVE6AP; FLJ26981; HPVE6A; CTCL tumor antigen se37-2; human papilloma virus E6-associated protein; oncogenic protein-associated protein E6-AP |
| Accession | Q05086 |
Fig.1. Human Ubiquitin-protein ligase E3A (UBE3A) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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