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Human Trinucleotide repeat-containing gene 6A protein (TNRC6A) ELISA Kit

Human Trinucleotide repeat-containing gene 6A protein (TNRC6A) ELISA Kit

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Specification

Product name Human Trinucleotide repeat-containing gene 6A protein (TNRC6A) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Trinucleotide repeat-containing gene 6A protein (TNRC6A) ELISA Kit employs a two-site sandwich ELISA to quantitate TNRC6A in samples. An antibody specific for TNRC6A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNRC6A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNRC6A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNRC6A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TNRC6A; CAGH26; DKFZp666E117; FLJ22043; GW1; GW182; KIAA1460; MGC75384; TNRC6; CAG repeat protein 26; EDIE; GW182 autoantigen; glycine-tryptophan protein of 182 kDa; trinucleotide repeat-containing gene 6A

Product Properties

Kit components • Human Trinucleotide repeat-containing gene 6A protein microplate
• Human Trinucleotide repeat-containing gene 6A protein standard
• Human Trinucleotide repeat-containing gene 6A protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Trinucleotide repeat-containing gene 6A protein (TNRC6A) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNRC6A. No significant cross-reactivity or interference between Human TNRC6A and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Trinucleotide repeat-containing gene 6A protein is a member of the trinucleotide repeat containing 6 protein family. The protein functions in post-transcriptional gene silencing through the RNA interference (RNAi) and microRNA pathways. The protein associates with messenger RNAs and Argonaute proteins in cytoplasmic bodies known as GW-bodies or P-bodies. Inhibiting expression of this gene delocalizes other GW-body proteins and impairs RNAi and microRNA-induced gene silencing. Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins.
Gene ID 27327
Alternative TNRC6A; CAGH26; DKFZp666E117; FLJ22043; GW1; GW182; KIAA1460; MGC75384; TNRC6; CAG repeat protein 26; EDIE; GW182 autoantigen; glycine-tryptophan protein of 182 kDa; trinucleotide repeat-containing gene 6A
Accession Q8NDV7

Image & description

Fig.1. Human Trinucleotide repeat-containing gene 6A protein (TNRC6A) Standard Curve.

Fig.1. Human Trinucleotide repeat-containing gene 6A protein (TNRC6A) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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