|Product name||Human Transmembrane protein 9B (TMEM9B) ELISA Kit|
|Applications notes||This Human Transmembrane protein 9B (TMEM9B) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM9B in samples. An antibody specific for TMEM9B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM9B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM9B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM9B bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Human Transmembrane protein 9B microplate
• Human Transmembrane protein 9B standard
• Human Transmembrane protein 9B detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Human Transmembrane protein 9B (TMEM9B) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMEM9B. No significant cross-reactivity or interference between Human TMEM9B and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||The intracellular signaling pathway by which TNF induces its pleiotropic actions is well-characterized and includes unique components, as well as modules shared with other signaling pathways. TMEM9B as an important component of TNF signaling and which is a module shared with the IL-1β and Toll-like receptor (TLR) pathways. is a glycosylated protein localized in membranes of the lysosome and partially in early endosomes. The expression of TMEM9B is required for the production of proinflammatory cytokines induced by TNF, IL-1β, and TLR ligands, but not for apoptotic cell death triggered by TNF or Fas ligand. TMEM9B is essential in TNF activation of both the NF-κB and MAPK pathways.TMEM9B is a key component of inflammatory signaling pathways and suggest that endosomal or lysosomal compartments regulate these pathways.|
Fig.1. Human Transmembrane protein 9B (TMEM9B) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.